Part:BBa_K3593006

ssDNA, capture probe of control line for RPA method

The capture strand composes the control line of our test strip, which is complementary to the reporter probe on Au nanoparticle. This allows the capture of Au particle at any time with a toxin or not, to verify the function of lateral flow assay.

Sequence and features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Characterisation of this part

Design

Besides aptamers, we have some short single stranded DNA known as 'probes', which binds to the reporter probe on Au nanoparticle. BBa_K3593006 is one of those so-called 'probes' in our design, in which it reports a red color anyway on the control line, to verify the function of lateral flow construction.[1]

References

1.Jauset-Rubio M, Svobodová M, Mairal T, et al. Aptamer Lateral Flow Assays for Ultrasensitive Detection of β-Conglutin Combining Recombinase Polymerase Amplification and Tailed Primers. Anal Chem. 2016;88(21):10701-10709. doi:10.1021/acs.analchem.6b03256